With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. 0. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Rutin was used to generate standard curves for quantification. ，高内涵成像分析技巧——3D功能展示1-3D预览、共定位、测距，SpectraMax Mini 多功能微孔板读板机带你自信地迈出第一步！ ，多功能酶标仪 SpectraMax i3x 之动力学检测法的设置，iD5注射器卡盒操作视频 - 20201228_8，SpectraMax iD 系列触控屏 overview，【网络讲堂】高内涵. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. Settings not required by a reader are indicated by ‘---’. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Microplate Readers SpectraMax. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Version 7. Download PDF. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. g. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Itemploysa. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. As. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Example: [Drawer]. Biochrom New EX Read 400 Microplate Reader. Unparalleled performance on a personalized platform. SpectraMax i3, i3x, and iD5 readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Figure 1. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Danaher Inc spectramax i3x. QuickSync. Western blot assay 5 x 105 cells were seeded in 24-well plates in 500 μl growth medium and stimulated with novel compounds at 10μM. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). GloMax Discover System. The protocols are also available on the SoftMax Pro Software protocol sharing. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析Spectramax i3x fluorescence plate reader to measure fluo-rescence resonance energy transfer (FRET) signals from cell lines expressing CFP-YFP FRET sensors for glucose and ATP, as these sensors give large changes in FRET ratio in response to appropriate conditions, and are expressed in cells at high levels. gen) on a Spectramax i3X luminometer. 세포신호전달은 세포가 환경에 반응하고 다른 세포와 소통할 수 있게 합니다…. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. Pancreatic lipase activity was determined by measuring the absorbance of the test solution at 405 nm with a micro plate reader (MD SpectraMax i3x). User-upgradeable detection modules allow you to expand the capabilities of your microp. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. SeeSpectraMax MiniMaxImagingCytometeronpage85. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 030 fmol. SpectraMax Quant dsDNA Assay Kits. Order Now. Table 1. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. Asterisks indicate the dilution of assay control that should be minimally detectable for each kit. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel detection capabilities without the need for service. D 1 1. Specifically, we present neurite. 蛍光 発光 ELISA. A: percent GFP-positive cells calculated using the classification feature in the software. ZERO BIAS - scores, article reviews, protocol conditions and more. Bioz Stars score: 98/100, based on 1 PubMed citations. . Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. After incubation at 37 °C for 1 h, absorbance was measured at 420 nm (SpectraMax i3x; Molecular Devices). *On the SpectraMax i3x reader only. Automated for ease of use. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. 314 low lamp level warning. ATP (“flash” luminescence using . The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 2 Parallel Line Analysis Conc log [ng/ML] RLU. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. EUROPE. +44 118 944 8000. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. 305 reference level saturation. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. Bioz Stars score: 98/100, based on 1 PubMed citations. 在这里，我们展示了如何使用SpectraMax®i3x和 SpectraMax®iD5多功能微孔板读板机应用HTRF技术进行 Gq-偶联受体的分析。G蛋白偶联受体的分析主要通过检测 cAMP和IP3这两条信号转导通路调控介导的细胞内钙离子水 平的升高。Cisbio公司的IP-One Gq试剂盒通过检. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. 315 can't find zero order. HTRF IP standard curve. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. i3x Specifications. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 1. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析 We would like to show you a description here but the site won’t allow us. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Serial number 33 900 01-3387. It allows you to explore cellular pathways and protein activation and expression in one system. Molecular Devices’ ScanLater Western Blot Detection Cartridge uses TRF detection to turn its SpectraMax i3X and Paradigm plate readers into Western blot readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. Tristar 5 Multimode Microplate Reader. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. The corrected absorbance is within 5% of the. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. STAKMAX. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. NORTH AMERICA. Average 98 stars, based on 1 article reviews. 0 mm hex key. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. 1. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. At each time point, the samples were shaken and fluorescence was measured for SEP (488-nm excitation and 530-nm emission) and. Antigen Quantitation Protocol. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. 1. We would like to show you a description here but the site won’t allow us. SpectraMax i3/i3x readers must be equipped with a suitableThe SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. Explore Multi-Mode Validation Plate that confirms instrument performance for spectramax i3, i3x, Paradigm system & FilterMax Reader. SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 800. As. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. Description 1. 00800 665 32860. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. Modularity does provide some measure of forward vision into upcoming or future experimental needs. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. Time-. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. 2-µL or 4-µL samples were pipetted onto a 64-well SpectraDrop slide in quadruplicate, following the plate map in Figure 2. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax i3 Multi-Mode Plate Reader enables characterization of proteins by measuring intrinsic tryptophan fluorescence. . 58% for the detection module and 2. Using optimized settings for the Transcreener FP assays presented in this application note, the SpectraMax i3x. Research Article AntioxidantActivity,PolyphenolicContent,andFT-NIR AnalysisofDifferentAspiliaafricanaMedicinalPlantTissues DenisOkello ,1,2,3YuseongChung,1HyoseonKim. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. For each kit, the reader was able to detect the lowest dilution as positive. . . The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. The calculated LOQ values were 0. Title: Fluorescence Intensity SpectraMax Paradigm or i3 Detection Cartridge | Molecular Devices Author: Molecular Devices, LLC Subject: Explore this datasheet to know more about Fluorescence Intensity Detection Cartridges for SpectraMax Paradigm and i3x, which provide speed, sensitivity, and flexibility & uncompromising performance for either top or. Curcumin binding assay. Promega. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. . A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Capture Flash Assays with Ease. The SpectraMax® i3x Multi-Mode Microplate Reader evolves with your future needs and offers an unlimited breadth of application possibilities. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. More Product Information. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. 0 mm hex key to remove the six blue retaining screws that secure the transport bracket (B) to the instrument. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Wokingham, Berkshire RG41 5TS United Kingdom. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. . The SpectraMax i3x reader features a cooled photomultiplier tube (PMT) for low background noise in luminescence. . I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. 001 0. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. no. Read modes. Sensitive TRF detection of two simple, mix-and-measure assay workflows. If no sound is heard during initialization, contact technical support. Controls containing all reaction. The dual auto injector module. ZERO BIAS - scores, article reviews, protocol conditions and more. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 7. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. Both Microplate Optimization and Read Height Adjustment were. The calculated LOQ values were 0. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Molecular Devices are known for both the reliability and the wide uses of their products. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 4. pathways and protein activation and expression in one system. Supplier: Molecular Devices. to View Figures. Manufacturers try to design their instruments so they can be. Measured using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. BMG LABTECH VANTAstar. The SpectraMax® i3x offers a wealth of applications; it can measure fluorescence, absorbance and luminescence, and its modular upgrades enable western blotting, imaging cytometry and injectors. Future ready The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. Molecular Devicesの全商品を見る. Tip: MCF-7 cells have a polygonal morphology with irregular dimensions, and they have a habit of clumping into large aggregates. SpectraMax i3Instrumenttovisuallyinspectyoursampleandtoruncell-basedassaysat cellularorwhole-cellresolution. 12911151) 0. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. 과 같은 Parameter를 검사하여 평가할 수 있습니다. 1 Microplate Data Acquisition & Analysis Software for Windows. . Supported Windows Operating System: See release notes. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. . **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 313 reference gain check fail. 660-665 Eskdale Road. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Published Dec 16, 2020 | Updated Oct 10, 2023. In this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. Concentrating the excitation light on the sample through label specific optics, high levels of sensitivity and performance are reached. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Price from $9. 99. Background Parallax,Columns 2. Microplate Reader Shelf. GM2000 Register or Sign In. 0001 0. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Note: Additional readers with similar performance are listed. Sufficient for 1000 reactions. 302 low ref light. Select PMT gain o PMT gain = energy used by detector to collect signal o Low gain for samples with high expected signalCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Figure 1. The SpectraMax i3x reader provides theThe SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. The Curcumin binding to p53Y220C was also measured using the emission maxima of Curcumin at 495 nm after. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. AlphaScreen. Downloads. Microplate optimization should be performed the first time the assay is run, and subsequently for . . The SpectraMax i2X is used for cell migration, cell. Get a front row view of your cells. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 1 and above:. 3. SoftMax Pro. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. com The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. TR-FRET ratios were calculated by dividing the RFU value for the 665-nm reading by the RFU value for the 616-nm reading, and the resulting numbers were multiplied by 1000. (SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. . The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. 98 / 100 stars. Prepare your i3/i3x instrument for shipment. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. . Bulk Kit. The MiniMax cytometer with SoftMax® Pro Software can be used to image and analyze a variety of cells. StackMax Microplate Handling System. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. Description 1. Facilities. Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. The dynamic range of the assay is about 3. Fluorescence measurements of the soil solutions and enzyme activities were made using a microplate reader (SpectraMax i3x, Molecular Devices, USA). Find our technical support information along with contact details. The treatments were. SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax MiniMax 300 Imaging Cytometer *Here, the NucView 488 with DMSO formulation was used. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. . ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. using the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. 306 plate air cal fail,low light. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. HeLa cells were plated at 15,000 cells per well in 100 µL. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. TaqMan SNP. . SpectraMax i3x, onboard luminescence 121. Find our technical support information along with contact details. Supplier: Molecular Devices. 2 Note Section Patch:1) Select the correct plate and the wells that you want to measure. 020 fmol 0. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. View the Molecular Devices website. Quantification of NETs was performed with the SpectraMax i3x microplate-reader (Molecular Devices, United Kingdom). The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. SpectraMax i3x and Paradigm readers require the FP-FLUO. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Expand your lab’s capabilities to include flash applications such as dual luciferase and ATP assays with the SpectraMax® i3x injector module with SmartInject® Technology ensuring equal mixing across the plate for high-precision experiments. Time Resolved Fluorescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Overview of Western Blot Cartridge. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Videos & Webinars. Shake intensity. Spectranet customer portal Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Prepare your i3/i3x instrument for shipment. 5 Figures from 202 Publications Thermo Fisher Scientific. Negative samples contained only MycoAlert Assay Buffer. Figure 4. ; The top 3 importers of Spectramax are India with 365. Dilution series of HeLa cells transfected with firefly (red plot) and Renilla (blue plot) luciferases. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringfluorescence polarization, trf, fret, igg quantitation, elisa, western blot, spectramax i3x, spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiterA microplate reader that can detect two or more applications is considered a multi-mode plate reader. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. In brief, a pre-titrated dose of virus was incubated with 8 serial 5-fold dilutions of serum samples in duplicate in. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viability Introduction. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. 01 0. ü; SpectraMax® Mini reader support. Find regional support. Description. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. +44-118-944-8000. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Expanded dynamic range Engineered for performance with spectral fusion illumination for increased sensitivity across the entire excitation range. Open the catalog to page 1. Fluorescent Polarization: Solid black plates, as above. Data from Analyst®, and FLIPR® systems can also be easily imported and analyzed. 1. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. . . Nuclear Receptor. Binding and Inhibition. It allows you to explore cellular pathways and protein activation and expression in one system. Results were plotted as ratio of green/red vs. SpectraMax i3x Multi-Mode Detection Platform. *On the SpectraMax i3x reader only. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. to View Figures. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence polarization, and time-resolved. 1. infoboxeu@moldev. 3 Figures from 612 Publications Promega. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. 304 signal level saturation. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Bioz Stars score: 98/100, based on 1 PubMed citations. The chamber. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 305 reference level saturation. It can be upgraded with. 7 mm for all shake intensities. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Multimode Readers Comparison Chart. Features. 9 x 10-15. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全.